h50 (that binds proteins through reverse-phase or hydrophobic interactions)  (Bio-Rad)

Journal: Proteome Science

Article Title: Enriched sera protein profiling for detection of non-small cell lung cancer biomarkers

doi: 10.1186/1477-5956-9-55

Figure Lengend Snippet: Comparison of low-MW predictor protein peaks intensities (H50) between NSCLC patients and controls

Article Snippet: In a preliminary study, in order to set up the experimental conditions, pooled serum samples were loaded onto three different types of ProteinChip Arrays (Bio-Rad Laboratories Inc., Hercules, CA, USA): H50 (that binds proteins through reverse-phase or hydrophobic interactions), CM10 (negatively charged surface that acts as a weak cation-exchanger) and IMAC30-Cu (Immobilized Metal Affinity Capture surface pre-activated with copper).

Techniques:

Journal: Proteome Science

Article Title: Enriched sera protein profiling for detection of non-small cell lung cancer biomarkers

doi: 10.1186/1477-5956-9-55

Figure Lengend Snippet: Serum protein profile of low-MW predictor peaks (H50) . Representative spectra (in duplicate) obtained by SELDI-ToF-MS analysis concerning the 5 statistically significant peaks detected with H50 ProteinChip Array and used as predictors to build the decision classification tree shown in Figure 4. The peaks of interest are highlighted in rectangles and their m/z values are reported above. (Ctrl = control subjects) .

Article Snippet: In a preliminary study, in order to set up the experimental conditions, pooled serum samples were loaded onto three different types of ProteinChip Arrays (Bio-Rad Laboratories Inc., Hercules, CA, USA): H50 (that binds proteins through reverse-phase or hydrophobic interactions), CM10 (negatively charged surface that acts as a weak cation-exchanger) and IMAC30-Cu (Immobilized Metal Affinity Capture surface pre-activated with copper).

Techniques:

Journal: Proteome Science

Article Title: Enriched sera protein profiling for detection of non-small cell lung cancer biomarkers

doi: 10.1186/1477-5956-9-55

Figure Lengend Snippet: Decision tree classification diagram of low-MW protein peaks from NSCLC patients and controls, using H50 conditions . The numbers in the root (top), descendant nodes (exagons) and terminal nodes (rectangles), represent the classes (NSCLC patients and controls, N = sum of NSCLC and controls). The numbers below the root and descendant nodes indicate the values of mass peak and its intensity, respectively.

Article Snippet: In a preliminary study, in order to set up the experimental conditions, pooled serum samples were loaded onto three different types of ProteinChip Arrays (Bio-Rad Laboratories Inc., Hercules, CA, USA): H50 (that binds proteins through reverse-phase or hydrophobic interactions), CM10 (negatively charged surface that acts as a weak cation-exchanger) and IMAC30-Cu (Immobilized Metal Affinity Capture surface pre-activated with copper).

Techniques:

Journal: Proteome Science

Article Title: Enriched sera protein profiling for detection of non-small cell lung cancer biomarkers

doi: 10.1186/1477-5956-9-55

Figure Lengend Snippet: Comparison of high-MW range predictor peak intensities between NSCLC patients and controls for IMAC30-Cu and H50

Article Snippet: In a preliminary study, in order to set up the experimental conditions, pooled serum samples were loaded onto three different types of ProteinChip Arrays (Bio-Rad Laboratories Inc., Hercules, CA, USA): H50 (that binds proteins through reverse-phase or hydrophobic interactions), CM10 (negatively charged surface that acts as a weak cation-exchanger) and IMAC30-Cu (Immobilized Metal Affinity Capture surface pre-activated with copper).

Techniques:

Journal: PLoS ONE

Article Title: Proteomic fingerprinting in HIV/HCV co-infection reveals serum biomarkers for the diagnosis of fibrosis staging

doi: 10.1371/journal.pone.0195148

Figure Lengend Snippet: SELDI-TOF MS biomarkers detected in HIV/HCV co-infection.

Article Snippet: Samples were randomized within and across arrays with blank spots included as negative controls and applied to three types of ProteinChip arrays: weak cation exchange (CM10), immobilized metal affinity capture (IMAC30) and hydrophobic/reverse-phase (H50) (all from Bio-Rad).

Techniques: